Table 2.
Average expression ratios (DS samples/controls) calculated from microarray and RT-PCR data and Pearson correlation coefficient (r) between microarray and RT-PCR data. Hsa21 genes are in bold.
| Functional Category | Probe ID | Gene Name | GenBank | Micro-array Fold change | Micro-array p-value | RT-PCR ratio | r | Primers for RT-PCR |
| Oxydative Phospho-rylation | 201226_at | NDUFB8 | NM_005004 | 0.65 | 0.0160 | 0.76 | 0.78 |
LEFT-GCCAAGAAGTATAATATGCGTGTG RIGHT-GTCAGGGAGCTTCGGGTAG |
| 201740_at | NDUFS3 | NM_004551 | 0.76 | 0.0294 | 0.60 | 0.88 |
LEFT-GATTATGGCTTCGAGGGACA RIGHT-ACCCGCTTCACTTCATCATC |
|
| 201903_at | UQCRC1 | NM_003365 | 0.77 | 0.0142 | 0.89 | 0.84 |
LEFT-CCGAGCAGTCCTCTCAGC RIGHT-TGTTCCCTTGAAAGCCAGAT |
|
| 201966_at | NDUFS2 | NM_004550 | 0.67 | 0.0010 | 0.58 | 0.89 |
LEFT-GAATGGGCACAGCAGTTTG RIGHT-GGCCCAAAGTTCAGGGTAAT |
|
| 203606_at | NDUFS6 | NM_004553 | 0.75 | 0.0464 | 0.63 | 0.84 |
LEFT-AGAAGGTCACGCACACTGG RIGHT-CACGGGCTGCTCTGCTAT |
|
| 203663_s_at | COX5A | NM_004255 | 0.79 | 0.0400 | 1.04 | 0.79 |
LEFT-AACTGGGCCTTGACAAAGTG RIGHT-GGTAACTGTTCACACTCAAGTAGCA |
|
| 203858_s_at | COX10 | NM_001303 | 0.77 | 0.0101 | 0.66 | 0.92 |
LEFT-CTTTTGACTGGCCCTGTTTC RIGHT-ACCAGCGGTCTGTTCTTTGT |
|
| 218160_at | NDUFA8 | NM_014222 | 0.73 | 0.0445 | 0.71 | 0.94 |
LEFT-GTCATGCCGGGGATAGTG RIGHT-TTAAGCACAGCAGAACTAATTTTCA |
|
| 218190_s_at | UCRC | NM_013387 | 0.76 | 0.0017 | 0.76 | 0.89 |
LEFT-GACGCTATCTACGACCACATCA RIGHT-GGTCCTTCTGGCCTGGAT |
|
| Extra-cellular Matrix | 201069_at | MMP2 | NM_004530 | 1.59 | 0.0069 | 1.89 | 0.93 |
LEFT-TCCACCACCTACAACTTTGAGA RIGHT-AACTTGCAGGGCTGTCCTT |
| 202310_s_at | COL1A1 | K01228 | 1.55 | 0.0333 | 5.86 | 0.90 |
LEFT-TGTTCAGCTTTGTGGACCTC RIGHT-CTGTACGCAGGTGATTGGTG |
|
| 202403_s_at | COL1A2 | NM_000089 | 1.60 | 0.0192 | 3.59 | 0.77 |
LEFT-CACATGCCGTGACTTGAGAC RIGHT-TAGCATCCATAGTGCATCCTTG |
|
| 202994_s_at | FBLN1 | Z95331 | 1.72 | 0.0271 | 2.38 | 0.89 |
LEFT-GCCACAAGTGCGAGAACAC RIGHT-TAGACGTTGGCACACTCCTG |
|
| 209081_s_at | COL18A1 | AF018081 | 1.57 | 0.0079 | 1.91 | 0.95 |
LEFT-GTGGCCCTCTACGTGGACT RIGHT-TCTGAGTCATCGCCTTCCTC |
|
| 213428_s_at | COL6A1 | AA292373 | 1.62 | 0.0493 | 2.20 | 0.78 |
LEFT-AGGAGACCCTGGTGAAGCTG RIGHT-AGGTCCTGGGGCTCCTCT |
|
| 209156_s_at | COL6A2 | AY029208 | 2.39 | 0.0002 | 2.18 | 0.79 |
LEFT-GACCTGGTCGCTGAGAAGTT RIGHT-GCCTTGTGGAAGTTCTGCTC |
|
| Other genes | 205548_s_at | BTG3 | NM006806 | 1.68 | 0.0134 | 1.62 | 0.83 |
LEFT-GAGGCAGTTGAGAGGTTTGC RIGHT-GAGTGAGCTCCTTTGGCAAG |
| 35776_at | ITSN1 | AF064243 | 1.72 | 0.0003 | 1.57 | 0.88 |
LEFT-GTGAGCGGCACTGATTTGT RIGHT-GATCATGCTTCGCTCTTTCC |
|
| 205593_s_at | PDE9A | NM_002606 | 2.05 | 0.0067 | 2.05 | 0.89 |
LEFT-CAGAACGCACTCCGTACAAA RIGHT-TGGGCTCTACCTGTCCACTT |
|
| 211065_x_at | PFKL | BC006422 | 1.42 | 0.0088 | 1.97 | 0.81 |
LEFT-GGTGGACCTGGAGAAGCTG RIGHT-TCCAGGCGGAGTCAATGT |
|
| 200642_at | SOD1 | NM_000454 | 1.16 | >0.05 | 0.65 | 0.83 |
LEFT-GCATCATCAATTTCGAGCAG RIGHT-CAGCCTCTGTATTATCTCCAA |
|
| 203405_at | DSCR2 | NM_003720 | 1.15 | >0.05 | 0.85 | 0.78 |
LEFT-AAAGACTCGGCGTGTTGTC RIGHT-GAATTGCTGGGATTTTCCAT |
|
| 202671_s_at | PDXK | NM_003681 | 1.12 | >0.05 | 1.00 | 0.98 |
LEFT-CATACAGAGCCACGTCATCC RIGHT-GCATAGCCTGTGTGGTTTGA |
|
| 202325_s_at | ATP5J | NM_001685 | 1.00 | >0.05 | 1.17 | 0.78 |
LEFT-TGTTTGGCTTCTGTCTCACC RIGHT-GGCTGACCGAATGACAGAG |
|
| 202749_at | WRB | NM_004627 | 1.17 | >0.05 | 1.25 | 0.85 |
LEFT-CTCAGCTTCGTGTTTGGATG RIGHT-ACTGTGGAGAGCTCCTGCTT |
|
| 209033_s_at | DYRK1A | D86550 | 1.53 | 0.0001 | 1.71 | 0.84 |
LEFT-GATATCATATGGGTCAGGTCATTTT RIGHT-CTGGACTGTAACATAACACAGTATGC |
|
| 208370_s_at | DSCR1 | NM_004414 | 1.25 | >0.05 | 4.35 | 0.83 |
LEFT-TTTGCTCAGACCTTACACATAGGA RIGHT-GGGAGGGGAGATCAGAAACT |
|
| 210555_s_at | NFATC3 | U85430 | 0.45 | >0.05 | 0.54 | 0.97 |
LEFT-CTTTGCAATGGCAAGAGGA RIGHT-GATGAGGCACAGGCAAAGAT |
|
| 217526_at | NFATC2 | AI478300 | 0.77 | 0.019 | 0.67 | 0.84 |
LEFT-GAGTTCACATCCCAGAGTCCA RIGHT-GAGCACTCGATGGGGTTAGA |
|