Figure 3. Synergistic Binding of Jra and Stat92E to the Attacin-A Promoter.

ChIP assays of the transcription factors indicated below using various mutants. SL2 cells were depleted of the transcripts by dsRNA treatment, as indicated in the top box, for 3 d. Then chromatin extracts were prepared before (−) or after 30 min (+) of LPS/PGN treatment. The amounts of Attacin-A promoter fragments co-precipitated with antibodies against the transcription factors indicated below the data were measured by real-time PCR. The levels were normalized by the input used in each ChIP assay and are shown with standard deviations. These experiments were repeated independently at least three times.