Figure 6. Relish Is Displaced from the Attacin-A Promoter by the Repressosome.

(A) Left panel: soluble chromatin extracts were prepared from SL2 cells with (15 min or 8 h) or without (0 min) LPS/PGN treatment, and immunoprecipitated with antibodies against Relish, Jun, or Stat92E as described in Figure 3. Right panel: double ChIP assays. The precipitates obtained from the first ChIP of cells with (15 min) or without LPS/PGN treatment were analyzed separately in a second ChIP with the antibodies indicated at the top (second ChIP). The amounts of Attacin-A promoter fragments co-precipitated with the indicated antibody are shown.

(B) The transcript levels of each target gene are shown under Luciferase, Relish, Jra, Stat92E, or Dsp1 knock-down conditions with LPS/PGN treatment (10 μg/ml; 1h). The averages and standard deviations of triplicates assays are shown.