Figure 7. Up-Regulation of Relish Target Genes in Repressosome Mutant Flies during Bacterial Infection.

(A) Transcript levels of LPS/PGN-induced Attacin-A in mutant flies. Wild type (w¹¹¹⁸) and mutant flies were infected with E. coli, and the levels of the Attacin-A transcript were measured by real time PCR analysis 24 h after infection. Total RNA from groups of five flies was pooled for the analysis. The averages and standard deviations of three independent assays are shown. Attacin-A was not expressed without bacterial infection in any of the mutants (unpublished data). Abbreviations for the mutant flies are as follows: w, w¹¹¹⁸; R, Rel/Rel; S/d, UAS-shStat92E/+; da-Gal4/+; S/d/R, UAS-shStat92E/+; da-Gal4/Rel; S/h, UAS-hs-shStat92E/UAS-hs-shStat92E; S/h/R, UAS-hs-shStat92E/+; Rel/+; J, Jra^1A109/CyO; J/R, Jra^1A109/+; Rel/+; D, Dsp1[EP355]/Dsp1[EP355]; D/R, Dsp1[EP355]/Y; Rel/+.

(B–G) Bacterial clearance assays. Wild type and mutants were injected with the same number of E. coli, and the number of live bacteria inside each injected fly was measured as described in Materials and Methods and represented by a dot on the graph. The bars represent mean of colony forming unit (cfu). The abbreviations used are as in (A). p-values were calculated by Student's t-test. *p < 0.01. **p < 0.05. ***p > 0.2.