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. 2007 Sep 4;5(10):e254. doi: 10.1371/journal.pbio.0050254

Figure 10. Non-Mapped HuRef Sequences Mapped to Coriell DNA Samples by FISH.

Figure 10

Sequences from the HuRef donor that had no match based on the one-to-one mapping or BLAST when compared to the NCBI Human reference genome were tested by FISH. Fosmids were used as probes and the experiments were run, using Coriell DNA, to confirm the localization of the contigs or to map contigs with no prior mapping information. Shown here are four representative results. (A) An insertion at 7q22 where the FISH confirmed the HuRef mapping, (B) FISH result confirming the mapping of a sequence extending into a gap at 1p21. (C) Localization of a contig with no prior mapping information to chromosomal band 1q42. (D) An example of euchromatic-like sequence with no prior mapping information, which hybridizes to multiple centromeric locations.