Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jul 24;4:76. doi: 10.1186/1743-422X-4-76

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007 Butler-Cole et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Coimmunoprecipitation of proteins that interact with ECTV-PH. His-tagged ECTV-PH was immunoprecipitated with mouse monoclonal anti-His antibodies and Protein G-Plus agarose along with any bound proteins from a BS-C-1 cell lysate. Proteins were separated by SDS-PAGE and stained with Coomassie blue. Lane 1, control immunoprecipitation; lysate contained no His-tagged ECTV-PH. Lane 2, proteins isolated from immunoprecipitation on cells expressing His-tagged ECTV-PH. Three bands at 16 kDa, 38 kDa and 84 kDa were excised from the gel and identified by mass spectrometry as indicated; a fourth band at 28 kDa was identified as a dimer of ECTV-PH in a western blot.