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Western blot to test for actin in coimmunoprecipitates using rabbit IgG anti-actin primary antibody. Lane 1, purified rabbit muscle actin showing an immunoreactive band at 42 kDa (positive control). Lane 2, starting cell lysate showing presence of actin. Lane 3, proteins that coimmunoprecipitated with ECTV-PH as described in Figure 2 showing absence of detectable actin.
