Table 2.

Comparison of residues important in actin, poly(L-proline) and PIP₂ binding in human profilin 1, human profilin 3 and ECTV-PH. Identical and functionally conserved residues are indicated with an asterisk

Residue in human profilin 1	Equivalent residue in human profilin 3	Equivalent residue in ECTV-PH	Function in human profilin 1
W3	W4	W5	Poly(L-proline) binding*
Y6	Y7	I8	Poly(L-proline) binding
W31	W32	L33	Poly(L-proline) binding
Y59	L60	-	Actin binding
V60	Q61	-	Actin binding
N61	A62	-	Actin binding
K69	R70	F67	Actin and PIP2 binding
S71	C72	I69	Actin binding
V72	V73	V70	Actin binding*
I73	I74	Y71	Actin binding
R74	R75	T72	Actin binding
E82	D83	T80	Actin binding
R88	R89	L86	Actin and PIP2 binding
K90	K91	G88	Actin and PIP2 binding
T97	A95	V92	Actin binding
N99	A97	P94	Actin binding
V118	V116	T113	Actin binding
H119	H117	S114	Actin binding
G121	G119	R116	Actin binding
L122	I120	E117	Actin binding
N124	N122	Y119	Actin binding
K125	K123	R120	Actin and PIP2 binding*
Y128	H126	R123	Actin binding
E129	E127	D124	Actin binding*
H133	G131	N128	Poly(L-proline) binding
L134	L132	V129	Poly(L-proline) binding*
R135	R133	R130	PIP2 binding*
R136	M134	A131	PIP2 binding
Y139	A137	N134	Poly(L-proline) binding