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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Dev Biol. 2007 Feb 21;305(1):377–387. doi: 10.1016/j.ydbio.2007.02.015

Figure 2.

Figure 2

(A) Western immunoblot analysis of zebrafish lysates demonstrating overexpression of dominant-negative IGF1R:GFP fusion protein (dnIGF1R:GFP). First lane, lysates from zebrafish embryos injected with synthetic mRNA encoding GFP only; second lane, lysates from zebrafish embryos injected with synthetic mRNA encoding dnIGF1R:GFP. Upper band in dnIGF1R:GFP corresponds to the predicted molecular weight (~60 kDa) of dnIGF1R:GFP after denaturation in reducing conditions. (B) Vasa immunostaining of PGCs (arrows) in zebrafish embryo overexpressing GFP only (control); (C) Vasa immunostaining of PGCs in zebrafish embryo overexpressing dnIGF1R:GFP fusion protein. Magnification (B–C), 200x. Mean PGC numbers in B and C are summarized in Table 1.