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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Dev Biol. 2007 Feb 21;305(1):377–387. doi: 10.1016/j.ydbio.2007.02.015

Figure 5.

Figure 5

Ectopic PGCs undergo apoptosis. (A) Vasa immunostaining, and (B) TUNEL analysis of ectopic PGCs in dorsal trunk of igf1rb-MO-injected embryo; (C; merged image) colocalization of Vasa-positive (arrows) and TUNEL-positive (arrowheads) cells confirms DNA fragmentation in ectopic PGCs. (D) Vasa-positive, but (E) TUNEL-negative PGCs in genital ridge (circled) of igf1rb-MO-injected embryo; images merged in (F). An absence of DNA fragmentation in genital ridge PGCs confirms survival of the subpopulation of PGCs that successfully migrate to the genital ridge. Anterior is to the left in all images; magnification 200x.