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Ectopic PGCs are rescued from apoptosis by overexpression of Bcl2l. (A) Vasa positive cells (arrows), and (B) rare TUNEL-positive cells (arrowheads) in dorsal trunk of igf1rb-MO/bcl2l mRNA co-injected embryo; (C; merged image) lack of colocalized Vasa and TUNEL signals (arrows, arrowheads) confirms absence of DNA fragmentation in ectopic PGCs (compare with Fig. 6A–C). Magnification 200x.
