. Author manuscript; available in PMC: 2008 Apr 30.

Published in final edited form as: Dev Biol. 2007 Feb 21;305(1):377–387. doi: 10.1016/j.ydbio.2007.02.015

Table 1.

Mean number of PGCs in zebrafish embryos after targeted knockdown of Type-1 IGF receptors using morpholino oligonucleotides (MO), or a dominant-negative IGF1R:GFP fusion protein (dnIGF1R:GFP). Sample size indicated in parentheses. N.D., not determined.

	Stage of Development

Treatment Group	Prim-5	18-somite (18S)
Wild-type (non-injected)	32.6 ± 0.9 (10)^a	N.D.
Control MO-a/MO-b	33.9 ± 1.1 (21)^a	34.6 ± 3.4 (10)
igf1ra-MO	33.1 ± 1.8 (9)^a	30.6 ± 0.5 (8)
igf1rb-MO	18.2 ± 2.2 (18)^b	32.9 ± 3.2 (10)
igf1ra-MO + igf1rb-MO	19.3 ± 2.8 (8)^b	32.7 ± 1.82 (15)
igf1rb-MO + bcl2l^*	28.8 ± 1.5 (20)^a	N.D.
dnIGF1R:GFP	16.0 ± 3.1 (9)^b	34.4 ± 2.6 (8)
GFP	35.9 ± 1.7 (20)^a	N.D.

Superscript letters denote significant differences among same-stage embryos (ANOVA, Tukey’s, P < 0.05). No significant differences were detected among means of 18S embryo groups.

mRNA encoding zebrafish Bcl2l.