Figure 4.

Acetylated nucleosomes are substrate for HDAC1p and HDAC3p. (A) [³H]Acetyllysine-labeled nucleosomes were incubated with anti-HDAC1 (Left) or anti-HDAC3 (Right) immunoprecipitated complexes in the absence or presence of TSA (400 nM). Reaction products were resolved on a 15% SDS/PAGE gel, and histones were visualized by staining with Coomassie blue (Lower) and by autoradiography after enhancement (Upper). The positions of the four histones H2A, H2B, H3, and H4 are indicated. (B) The autoradiogram was quantified after scanning by using the image analysis program (National Institutes of Health). Results are expressed as arbitrary absorbance values.