Fig. 1.

Deletion of Atg7 specifically in Purkinje cells caused progressive dystrophic swelling of axon terminals. (A) Immunohistochemistry of Atg7 protein expression in Purkinje cells of Atg7^flox/flox and Atg7^flox/flox;Pcp2-Cre mice at P15 and P19. The endogenous Atg7 protein was present at P15 but absent at P19 in the Atg7^flox/flox;Pcp2-Cre Purkinje cells. (Scale bar: 100 μm.) (B) Progression of the abnormal Purkinje cell axon terminal swellings in the DCN of Atg7^flox/flox;Pcp2-Cre mice (anti-calbindin immunofluorescent staining in green with anti-NeuN counterstained in red) at P19, P35, and P56. Atg7^flox/flox was used as control. (Scale bar: 20 μm.) n = 3–5.