Fig. 2.

The axonal dystrophic swellings of the Atg7-deficient Purkinje cells contained no GFP-LC3 labeled autophagosomes but accumulated p62/SQSTM1. (A) The absence of GFP-LC3 puncta in Purkinje cell axonal dystrophic swellings (b and c) and somata (f and g) of Atg7^flox/flox;Pcp2-Cre/GFP-LC3 mice (P35). GFP-LC3 puncta were found in GFP-LC3/Lurcher Purkinje cell axonal dystrophic swellings (d) and somata (h) (P12). DCN (a) and Purkinje cell layer (PCL) (e) of control mice Atg7^flox/flox/GFP-LC3 are shown. (Scale bars: a, b, e, and f, 20 μm; c, d, g, and h, 10 μm.) (B) Anti-p62/SQSTM1 immunofluorescent staining (in green) showed accumulation of p62/SQSTM1 in Purkinje cell axonal dystrophic swellings (calbindin labeling in red) (white arrows) in the DCN of Atg7^flox/flox;Pcp2-Cre mice at P56. Atg7^flox/flox was used as control. (Scale bar: 10 μm.)