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Insertions per mutant ES cell clone
| No. of insertions per clone | ES clones, % |
|---|---|
| 1 | 38 |
| 2 | 32 |
| 3 | 12 |
| 4 | 8 |
| 5 | 6 |
| 6 | 1 |
| 7 | 3 |
Purified clones with insertions in GPCR genes (total of 105 characterized clones) were checked for the total number of the vector insertions by quantitative slot-blot hybridization. We found up to seven insertions per mutant clone.
