Fig. 4.

Rep-ori replication specificity. Cultures of RN4220 strains containing the pri-rep-ori modules of SaPIbov1 and SaPI2 and chimeric constructs as shown were grown on CY broth at 43°C until OD₅₄₀ = 0.6. Samples were removed and used to prepare minilysates. Lysates were separated by agarose gel electrophoresis and probed with a probe specific for the carrier plasmid, pRN9220. Above the blot is shown the composition of the pri-rep-ori module of each of the plasmids tested. Black indicates segments derived from SaPIbov1, gray, from SaPI2. E indicates empty vector.