Fig. 5.

SaPIbov1 pri-rep-ori subclone stability. Bacteria RN4220 containing the SaPIbov1 pri-rep-ori subclone grown on selective medium were grown on CY broth without antibiotic. Samples were taken every 30 min during 3 h and plated on GL plates with or without Em. The graph shows the number of EmR colonies obtained in relation to the total number of colonies obtained during the 3 h (solid line). The dashed line represents a plasmid that does not replicate. The dotted line represents a stable plasmid.