Fig. 3.

Characterization of captured vessel and nonvessel elements. (a) Images of vessel and nonvessel elements are marked by using the LMPC system. (b) Quantification of vessel-specific mRNA from 400,000 μm² of vessel elements (VE) or nonvessel elements (non-VE) captured following laser microdissection from RNALater treated, UEA I stained and dehydrated human wound tissue. RNA was extracted from the captured tissue, amplified, and reverse transcribed into cDNA. Gene expression was quantified by using real-time PCR (normalized to β-actin). *, P < 0.05 compared with vessel element. SMA, smooth muscle actin; KRT14, keratin 14; VIM, vimentin.