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. 2007 Jun 1;115(9):1325–1332. doi: 10.1289/ehp.9874

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Sample ratio intensity plot in MM6 cells. The dashed line indicates the median. Upper and lower outer fences of 300 spotting-buffer quadruples (arrow) were calculated according to Tukey and Aase (1977). Oligonucleotides of 10 Arabidopsis and Shinorizobium genes were synthesized and added to the probes in concentration of 200–10,000 fg (spiking controls). Spiking controls were reproducibly separated from buffer spots, whereas randomized negative controls (i.e., oligonucleotides that do not bind human RNA) were always within the calculated fences (not visible due to high spot density). Genes (rhombi) outside the fences were regarded as differentially transcribed. The y-axis is the dual logarithm of mRNA spot intensity after indoor dust exposure divided by spot intensity after control exposure; the x-axis is the geometric mean of spot intensity (arbitrary scale).