Multiple sequence alignment of TS with MJ0757. Optimal alignment of TS sequences B. subtilis, E. coli, S. cerevisiae, L. casei, and MJ0757 was generated with clustal w (27), using default parameters. The catalytic Cys and other residues from Table 1 are highlighted in yellow. The 17 absolutely conserved residues are annotated by an asterisk, and other strongly conserved residues are marked by a dot.