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A genetic system for the isolation and characterization of site-specific proteases. (Left) The infecting phage encodes a random β-gal:cDNA fusion protein that has no effect on the steady-state levels of the target repressor containing the proteolytic processing site (PPS). (Right) The infecting phage encodes the appropriate site-specific protease that cleaves the recombinant repressor, ultimately resulting in lytic replication.
