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Example of a typical force-extension curve of the 20-basepair DNA duplex (DNA20s). The force-extension curve shows a rupture event of a hybridized 20-basepair DNA duplex, recorded at a retract velocity of 1007 nm/s. The DNA duplex dissociates at a force of 53 pN, without showing any evidence of the B-S transition (see inset). The force-extension curve of the PEG-DNA complex follows the two-state FJC-fit (black) without possessing a measurable deviation.
