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Experimental RR spectra of the Pr state of the 65-kDa fragment of phyA reconstituted with the (A, solid line) unlabeled and (B, dotted line) 13C(5) PCB measured in H2O. Trace B represents difference of the spectra in A (nonlabeled minus 13C(5)). The corresponding difference spectra obtained from the calculated Raman spectra are shown in C (protonated ZZZasa) and D (protonated ZZZssa).
