TABLE 2.
Decomposition of pHLIP tryptophan fluorescence spectra in the absence and presence of POPC liposomes at pH values 8.0 and 4.0
| Lmax, nm | F, % | Ksv, % | |
|---|---|---|---|
| pHLIP, pH8.0 | 325.0 ± 1.2 | 9.5 ± 3.7 | 18.1 ± 7.8 |
| 349.1 ± 0.5 | 90.5 ± 3.7 | 81.8 ± 0.8 | |
| pHLIP + POPC, pH8.0 | 326.4 ± 1.1 | 39.4 ± 3.0 | 16.1 ± 1.7 |
| 347.4 ± 1.5 | 60.6 ± 3.0 | 80.1 ± 5.1 | |
| pHLIP + POPC, pH4.0 | 321.8 ± 4.2 | 21.6 ± 1.0 | 9.9 ± 1.7 |
| 339.1 ± 4.6 | 78.4 ± 1.8 | 20.4 ± 1.9 |
The table contains the parameters of log-normal spectral components: the position of each spectral maximum (Lmax, nm), the total intensity contribution of each component (F, %), and the relative Stern-Volmer constant (Ksv, %, where 100% corresponds to the value of the Stern-Volmer constant for free tryptophan emission quenched by acrylamide in aqueous solution, which was taken as 16.8 M−1).