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. Author manuscript; available in PMC: 2008 Jan 26.
Published in final edited form as: Cell. 2007 Jan 26;128(2):341–355. doi: 10.1016/j.cell.2006.11.048

Figure 3.

Figure 3

Direct stimulation of purified GC by purified PAK. A, Coomassie blue staining of purified recombinant proteins. B, Direct stimulation of GC-E-intra by PAK. The activity of purified GST-GC-E-intra was assayed in vitro in the presence or absence of purified GST-PAK1, GST-PAK2, GST-PAK1(K298A), GST-PAK2(K278A), and/or Rac1-GTPγS. Data are shown as mean ± s.d. of three experiments. **, P < 0.001; *, P < 0.005; ++, P < 0.01 (Student’s t-test). C. Dose-response curve of the activation of purified GST-GC-E-intra by purified GST-PAK2 in the presence of Rac1-GTPγS.

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