Figure 1.

Effect of BK and B16F10-Nex2 tumor cells on pro-angiogenic closed structures formed by sprouting of endothelial cells. HUVECs were plated on Matrigel in medium supplemented with 0.2% of FCS in the presence of BK, B16F10-Nex2 supernatant, cell membrane or lysate and irradiated B16F10-Nex2 whole cells. The number of pro-angiogenic structures was counted after 18 h. One representative picture of four different treatments is shown with the respective counts. * p < 0.005 vs control; ** p < 0.0005 vs control.