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HPLC analysis of FRET peptides degradation by the B16F10-Nex2 supernatant. Abz-GFSPFR-EDDnp (A, C) or Abz-GFSPFRQ-EDDnp (B, D) were incubated with recombinant oligopeptidase TOP (A, B), or B16F10-Nex2 supernatant (C, D) in 50 mM Tris-HCl pH 7.4 at 37°C. Reaction products were separated by HPLC and were identified by mass spectrometry. Chromatograms developed by fluorescence detection at λem. = 420 nm and λex. = 320 nm.
