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HPLC analysis of BK degradation by B16F10-Nex2 supernatant. BK (20 μM) was incubated for 1 h at 37°C with recombinant TOP (A) or B16F10-Nex2 supernatant (B) in 50 mM Tris-HCl, pH 7.4. The reaction products were separated by HPLC. Bradykinin fragments were determined by mass spectrometry.
