Table 2.
Effect of inhibitors on the hydrolysis of Abz-GFSPFRQ-EDDnp by B16F10-Nex2 supernatant and recombinant TOP and Neurolysin
| Inhibitors | Relative hydrolysis (%)1 | ||
| B16F10-Nex2 | rTOP | rNeurolysin | |
| Control (no inhibitor) | 100 | 100 | 100 |
| o-phenanthrolin (4 mM) | 0 | 0 | 0 |
| JA-2 (3 μM) | 2.6 ± 2.1 | 0.6 ± 1.2 | 1 ± 1 |
| Pro-Ile (1 mM) | 60 ± 6 | 87 ± 5 | 52 ± 4 |
| PMSF (0.1 mM) | 94 ± 6 | 91 ± 4 | 93 ± 4 |
| E64 (0.1 mM) | 88 ± 3 | 98 ± 2 | 99 ± 2 |
| Leupeptin (0.2 mM) | 81 ± 6 | 96 ± 2 | 96 ± 4 |
| Z-Pro-Prolinal (1 μM) | 93 ± 1 | 88 ± 3 | 92 ± 4 |
| Captopril (20 μM) | 92 ± 3 | 97 ± 2 | 98 ± 2 |
1100% hydrolysis represents the cleavage of 20 μM of Abz-GFSPFRQ-EDDnp by B16F10-Nex2 supernatant and recombinant enzymes during 5 min at 37°C in 50 mM Tris-HCl, pH 7.4. The values are means of three independent experiments.