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. 1998 Mar 17;95(6):2834–2837. doi: 10.1073/pnas.95.6.2834

Figure 4.

Figure 4

Ab389 binds to p53 from UV-treated cells. RKO cells (a) and early passage rat embryo fibroblasts (b) were treated with 10 J/m2 (30 J/m2 for RKO cells) and metabolically labeled with [S35]methionine and [S35]cysteine for 30 min, 24 h (2 h for RKO cells) after radiation. Cells were harvested and lysed as described (14). Extracts from untreated (lanes 1 and 3) and UV-treated (lanes 2 and 4) cells were used for immunoprecipitation with pAb421 (lanes 1 and 2) or Ab389 (lanes 3 and 4) followed by SDS/PAGE and autoradiography. (c) RKO cells were treated with 6 Gy of γ radiation. Four hours after radiation, cells were metabolically labeled and harvested as described above. Extracts from these cells were used for immunoprecipitation with pAb421 (lanes 1 and 2) or Ab389 (lanes 3 and 4) followed by SDS/PAGE and autoradiography.