Figure 4.

Influence of atRSZ33 overexpression on the embryo and seedling development. (A) Wild-type seed with embryo at globular stage. (B) 35S:gatRSZ33 seed, containing twin embryos indicated by arrows 1 and 2. (C and D) Close-up of embryos shown in B. (E and F) Close-up of wild-type and 35S:gatRSZ33 embryos at globular stage, respectively, showing abnormal division in suspensor (arrow in F). (G and H) Wild-type and transgenic embryos at heart stage. (I) Eight-day-old wild-type seedling. (J, M, and N) Eight-day-old transgenic seedlings. (K) Three-day-old transgenic seedling. (J) Twin seedlings, one of them with single cotyledon. (K) Equally developed twin seedlings. (L and M) Seedlings with single cotyledon. Arrows in J, L, and M point out shoot apical meristems. Bars, 50 μm (A–H) and 1 mm (I–N).