Fig. 2.
The studied glycolipids promote the in vitro expansion of human iNKT cells. Human CD56+ cells (2 × 106; NK/NKT mixtures) were cultured with 4 × 105 autologous immature DCs pulsed with the indicated glycolipid at 3 μg/ml on day 2 for 18 h. The percentage of CD161+/Va24TCR+ cells in the NK/NKT mixtures was gated by flow cytometry on day 9, and the total number of Va24i NKT cells is shown. Assays were performed in triplicate, and data are presented as mean ± SD. Similar results were obtained in three independent experiments. ∗, P < 0.05, compared with DMSO; #, P < 0.05, compared with C1.