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. 2007 Jun 12;104(25):10299–10304. doi: 10.1073/pnas.0703824104

Fig. 4.

Fig. 4.

Expansion and activation of murine splenocytes after injection of glycolipids. Spleens from BALB/c mice (n = 4 per group) were harvested 72 h after i.v. injection of glycolipids. The spleens of mice injected with 100 μl of 1% DMSO appear grossly normal. There is no significant difference in subpopulation of splenocytes between normal untreated and DMSO-treated mice. (A) Total number of nucleated cells and spleen size. (B–D) Population of innate immune cells including mature DCs (CD11C+/CD80+/CD86+) (B), activated NK cells (U5A2-13Ag+/CD3−/CD69+) (C), and activated NKT cells (U5A2-13Ag+/CD3+/CD69+) (D). Assays were performed in duplicate, and data are shown as mean ± SD. ∗, P < 0.05, compared with DMSO; #, P < 0.05, compared with C1.