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. 2007 Jun 11;104(25):10346–10351. doi: 10.1073/pnas.0703876104

Fig. 2.

Fig. 2.

Interactions of CLIP-170 CAP-Gly domains with MT/tubulin and EB1. (a) Pull-down assays of tubulin with GST-CAP-Gly domains. To show clear visible tubulin bands, the applied amounts of proteins on the gel were 1:4:2 for CAP-Gly-12:CAP-Gly-1:CAP-Gly-2. (b) Pull-down assays of CAP-Gly domains with GST-EB1. (c) Pull-down assays of tubulin with GST–CAP-Gly-2 mutants. Wild-type and mutant GST–CAP-Gly-2 domains were immobilized on the resin and tubulin was used as an input. Labels for conserved lysines are bold and underlined. (d) Pull-down assays of EB1 with GST–CAP-Gly-2 mutants. (e) Cosedimentation experiments of CAP-Gly-2 domains with MT.