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. 2007 Jun 11;104(25):10346–10351. doi: 10.1073/pnas.0703876104

Fig. 3.

Fig. 3.

Solution structure of the CAP-Gly-2–peptide complex. (a) A best-fit superimposition of the final 20 simulated annealing structures of the CLIP-170 CAP-Gly-2 domain (residues 212–281) bound to the α3-tubulin peptide (447–451) with the lowest energies is displayed. The CAP-Gly-2 domain backbone (cyan) and selected side chains (blue) are depicted with the peptide (magenta). (b) Close-up view of the hydrogen bonds (dotted lines) formed in the complex along the arrow in a (Right). The side chain of Phe-236 is omitted for clarity. (c) Pull-down assay of mutated CAP-Gly-2 domains with the α3-tubulin peptide suggests a critical role for Asn-253 in peptide binding.