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The CAP-Gly binding to the α2-tubulin peptide (the C-terminal α2-tubulin peptide shown in Table 1)
| Protein | Domain | Residues* | Kd, μM |
|---|---|---|---|
| Wild | |||
| p150Glued | CAP-Gly | 403 ± 42 | |
| CLIP-170 | CAP-Gly12 | 0.513 ± 0.005 | |
| CAP-Gly1 | 70.1 ± 0.5 | ||
| CAP-Gly2 | 3.79 ± 0.01 | ||
| Mutants | |||
| CLIP-170 | CAP-Gly2 | K224A | 53.7 ± 0.4 |
| K238A | 7.01 ± 0.03 | ||
| K252A | 124 ± 6 | ||
| K268A | 6.86 ± 0.05 | ||
| K277A | 26.0 ± 0.1 | ||
| R297A | 8.9 ± 0.1 | ||
| R298A | 7.7 ± 0.1 | ||
*Conserved basic residues are shown in bold with underlining.
