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. 2007 Jun 6;104(25):10358–10363. doi: 10.1073/pnas.0610365104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 2. — The in vivo methylation specificity of hybrid proteins. (A) The in vivo methylation specificity of hybrids between AloI and PpiI. Recombinant plasmids were digested with AloI or PpiI. (B) The in vivo methylation specificity of Tst-PpiTRD1. DNA of plasmid pTst-PpiTRD1 was digested with: lane 1, no enzyme; lane 2, PpiI; lane 3, TstI; lane 4, BglII; lane 5, BglII + HindIII; lane 6, BglII + Mph1103I. (C) Restriction map of the pTst-PpiTRD1 plasmid. Modified BglII target is underlined. L, DNA ladder.