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Kinetic constants of Polη for incorporation of the initiating nucleotide
| WRN | Km, μM | Vmax, % extension min−1 |
|---|---|---|
| (−) | 1.5 ± 1.0 | 22 ± 7.2 |
| (+) | 2.0 ± 0.8 | 59 ± 8.5 |
The apparent Km and Vmax values (mean ± SD) were calculated from Hanes–Woolf plots of measurements from three independent experiments carried out with DNA primer-template formed by annealing oligonucleotides 8 and 9.
