Fig. 3.

Concentration-dependent P-cluster maturation on Av1^ΔnifZΔnifB. P-cluster maturation assays were performed as described in Materials and Methods, except that the concentration of NifZ was varied between 0 and 20 μM (A), and the concentration of Av2^wt was varied between 0 and 104 μM (B). The maximal activities were reached at ≈2 μM NifZ (A) and 2.5 μM Av2^wt (B), respectively. The data presented here represent the average of three independent experiments. The error bars are indicated.