Figure 2.

The TMD of Sec71p contains a signal for ER localization. Various chimeras between Mfα1p, Wbp1p, and Sec71p were constructed as depicted on the left and expressed in the wild-type (SNY9), Δrer1 (SKY7), and ret1-1 (SKY27) cells. The transformants were examined for α-factor secretion by the halo assay. Note that a smaller halo indicates less secretion of α-factor, in other words, good ER localization of the chimeric protein. MW71WS (arrowhead) shows good ER localization in the wild-type but not in the Δrer1 or ret1-1 mutant.