Fig. 4.
CRT and Hsp90 have independent roles in ERAD of IR. (A) 293 cells expressing wild-type hIR were transfected with an empty vector or with CRT cDNA and treated with carrier or 4.5 μM GA for 20 h. Cell lysates were processed as described previously. (B) Cells stably expressing wild-type hIR were transfected with an empty vector or with Hsp90 cDNA and treated with either carrier or 50 mM 2DG and lysates were processed as described previously. (C) Cells were transfected with hIRmut cDNA alone or with Hsp90 cDNA and lysates were processed as described previously.