Fig. 2.

Mapping of the SALL4 functional site within the Bmi-1 promoter region by a luciferase reporter gene assay. In HEK-293 cells, 0.3 μg of different length Bmi-1-Luc constructs was cotransfected with 0.04 μg of Renilla luciferase plasmid and 0.9 μg of either SALL4A or SALL4B plasmid. P1254 and ^ΔP683 refer to Bmi-1 mutant promoter constructs, −1254 or −683, in which the −270 to −168 sequence was deleted. (A) Deletion constructs of the Bmi-1 promoter and their corresponding promoter activity stimulated by either SALL4A or SALL4B. (B) SALL4A and SALL4B stimulation of −1254 and −683 or P1254 and ^ΔP683 Bmi-1 promoter constructs.