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. 2007 Jun 5;104(25):10655–10660. doi: 10.1073/pnas.0610811104

Fig. 3.

Fig. 3.

Prolonged spinal delivery of LHVS reverses tactile allodynia and attenuates microglia activation in neuropathic rats. rrCatS is pronociceptive in naïve rats. LHVS (30 nmol per rat per day) intrathecally delivered from day 0 to day 7 after injury (horizontal black bar) does not modify allodynia after 3 and 5 days but reverses allodynia after 7 days of treatment (A). LHVS delivered from day 7 to day 14 after injury (horizontal black bar) reverses established mechanical allodynia (B) and attenuates microglia activation (OX42-ir) (D) as compared with vehicle (C). (C and D) Ipsilateral dorsal horn (left) and contralateral dorsal horn (right) and high-power images (Insets: 20× magnification; scale bars: 50 μm) of the ipsilateral dorsal horn. (Scale bars: 100 μm.) (E) Quantitative analysis of OX42-ir. ∗∗∗, P < 0.001 versus vehicle group, four rats per group. (F) Intrathecal injection of activated rrCatS- (0.3–3 μg per rat) induced mechanical hypersensitivity in naïve rat hind paws, whereas nonactivated rrCatS (1 μg per rat) is ineffective. (G) Intrathecal activated CatB and CatL do not alter mechanical thresholds. (H) Intrathecal LHVS (50 nmol per rat, 30 min before CatS) prevents the hyperalgesia evoked by intrathecal activated rrCatS (1 μg per rat). ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001 versus vehicle group in A, B, and F; versus LHVS + rrCatS group in H, six to eight rats per group.