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. 2007 Jun 5;104(25):10655–10660. doi: 10.1073/pnas.0610811104

Fig. 4.

Fig. 4.

CatS incubation reduces the levels of sensory neuron-associated FKN. (A and B) DRG neurons in culture express FKN immunoreactivity (FKN-ir) (green) on the cell membrane (arrows) as well as intracellularly (asterisks). (C and D) Incubation with rrCatS (10 nM) for 30 min reduces FKN-ir (green) (arrowheads). (E and F) Inactivated (heat-denatured) CatS (10 nM) does not significantly reduce FKN-ir (green). (G) Quantitative analysis of FKN-ir in cultured DRG, three independent experiments. ∗∗, P < 0.01 versus vehicle. (H) Quantitative analysis of FKN protein in cultured DRG extracts by using ELISA. Activated rrCatS was incubated for 30 min. Data represent three independent experiments (two rats per experiment). ∗, P < 0.05 versus vehicle. (I) Quantitative analysis of membrane and intracellular FKN-ir at the midplane of cultured DRG (e.g., K and N). Data represent three independent experiments. ∗, P < 0.05. (J–O) A series of z-stack images of DRG neurons incubated without rrCatS (J–L) or in the presence of activated rrCatS (M–O). Images were taken with the Zeiss Axioplan 2 fluorescence microscope via a Zeiss ApoTome system. DAPI (blue) was used as a nuclear marker for all of the panels. (Scale bars: 10 μm.) I-CatS, inactivated CatS.