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. 2007 Jun 11;104(25):10720–10725. doi: 10.1073/pnas.0701742104

Fig. 3.

Fig. 3.

Identification and characterization of the TAL effector genes pthXo6 and pthXo7. (A) Northern blot hybridization analysis of OsTFX1 induction by ME1 (pHM1) (empty vector) and ME1 (pthXo6). Induction of OsTFX1 was examined 24 h after treatment of rice leaves. Lane assignments are as follows: ME1 (pHM1), empty vector; ME1 (pthXo6), single pthXo6 gene in pHM1 vector. (B) Northern blot hybridization analysis of OsTFIIAγ1 induction by ME10 (pHM1) and ME10 (pthXo7). Induction of OsTFIIAγ1 was examined 24 h after treatment of rice leaves. Lane assignments are as follows: ME10 (pHM1), empty vector; ME10 (pthXo7), single pthXo7 gene in pHM1 vector. Ethidium bromide-stained total RNA (tlRNA) is shown Lower. (C and D) Substitutions in the NLS and AD mutations of PthXo6 or PthXo7, respectively, result in loss of OsTFX1 and OsTFIIAγ1 induction. Northern blot hybridization analysis was performed at 24 h after inoculation as described in Fig. 1. Strain assignments in C are ME1 with the following genes: WT, wild-type pthXo6; NLS−, pthXo6M123 lacking consensus NLS sequences; NLS-SV40, pthXo6M123 with SV40 NSL sequence. Assignments in D are ME10 with the following: NLS−, pthXo7M123 lacking NLS sequences; NLS-SV40, pthXo7M123 with SV40 NLS sequences; Water, mock-inoculation; WT, wild-type pthXo7.