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p27 and Spy1 colocalize in the nucleus. COS-1 cells were transiently transfected with p27, myc-Spy1, or p27 plus myc-Spy1. (A) p27 localizes to the nucleus as shown in panel a. Similarly, myc-Spy1 localizes in the nucleus as shown in panel c. (B) Cells transfected with both p27 and myc-Spy1 demonstrate that both proteins localize to the nucleus (panel c). Hoechst dye was used to stain the nucleus.
