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p27 interacts with Spy1 and CDK2. 293T cells were transfected with the indicated constructs. The lysates were immunoprecipitated with α-p27 or α–CDK2 sera and immunoblotted for Spy1, CDK2, and p27. Lane 8 demonstrates that flag-Spy1 (top panel), endogenous CDK2 (middle panel), and p27 (bottom panel) can coimmunoprecipitate. The top panel of lane 11 shows that Spy1 interacts with CDK2 and that this interaction is increased when p27 is overexpressed as demonstrated in lane 12.
