Skip to main content
. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Dev Cell. 2007 May;12(5):699–712. doi: 10.1016/j.devcel.2007.03.014

Fig. 1. Subcellular localization of endogenous GEF-H1 during mitosis.

Fig. 1

(A)GEF-H1 is associated with the spindle apparatus throughout mitosis. Asynchronous HeLa cells were fixed with methanol/acetone and quadruple-stained for endogenous GEF-H1 (red channel), tubulin (green channel), actin (blue channel) and DNA (yellow). Scale bar represents 20 μm.

(B) GEF-H1 localizes to the tips of cortical MTs. Asynchronous HeLa cells were extracted for soluble tubulin, fixed as above and stained using anti-pGEF-H1 (green), anti-tubulin (red) antibodies or CREST serum. Boxed regions are shown in higher magnification. Confocal micrographs (single slices of 0.54 μm thickness) were analyzed for colocalized data points (displayed as white overlays in the colocalization panel).

(C) During cytokinesis, GEF-H1 forms an equatorial ring encompassed by contractile actin structures. Confocal microscopy of mitotic HeLa cells stained with anti-GEF-H1 (green) and anti-actin (red) antibodies. Indicated regions (white arrows) are shown in higher magnification in the inserts.