Figure 7.

Enlargement of the early endosome in STAM2-overexpressing cells. (A–D″) COS-7 cells were transfected with FLAG-tagged full-length STAM2 (A–A″), STAM2ΔVHS (B–B″), N-terminal region (1–192) (C–C″), or STAM2ΔUIM (D–D″). The cells were double stained with anti-FLAG (A, B, C, and D) and anti-Hrs (A′, B′, C′, and D′) antibodies. A″, B″, C″, and D″ are merged images. Arrows in A′, A″, D′, and D″ indicate enlarged early endosomes in STAM2- and STAM2ΔUIM-overexpressing cells. Arrows in B′, B″, C′, and C″ indicate STAM2ΔVHS- and N-terminal region (1–192)-overexpressing cells with early endosomes of normal size. The nucleus (N) is also indicated (A′ and A″). Bars, 20 μm. (E) Proportion of cells exhibiting enlarged early endosomes among those overexpressing the indicated STAM2 deletion mutants. Mean ± SD of three independent experiments are shown.